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KMID : 0613820080180050660
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Journal of Life Science
2008 Volume.18 No. 5 p.660 ~ p.667
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Influence of Ammonium against Cadmium Effect on in vitro Growth, Chlorophyll and RubiscojRubisco Activase in Tobacco Leaves
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Cho Eun-Young
Roh Kwang-Soo
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Abstract
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Influence of ammonium against cadmium (Cd) effects on in vitro growth, chlorophyll and rubisco/rubisco activase was investigated in tobacco grown on M5 medium. In vitro growth reduced by Cd was recovered by NH4NO3, and this recovery was most significant when tobacco was treated with 80 mM NH4NO3. Chlorophyll levels were also recovered by 80 mM NH4NO3. Rubisco content at 80 mM NH4NO3 was significantly increased than that at other treatments. Rubisco activity showed patterns of change similar to rubisco content. These data suggest that rubisco content was associated with activity of rubisco, and that the content and activity of rubisco reduced by Cd were recovered by ammonium. The degree of intensity of 55 and 15 kD polypeptides identified as the large and small subunit of rubisco by SDS-PACE analysis at 80 mM NH4NO3 was significantly higher than that at other treatments. Under the assumption that effects of ammonium on rubisco may be related to rubisco activase, its content and activity were determined. The rubisco activase content at 80 mM NH4NO3 was more increased than other treatments. A similar change pattern was also observed in activity of rubisco activase.
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KEYWORD
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Ammonium, cadmium, chlorophyll, rubisco, rubisco activase
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